Voice in sight!


Published in 2018 in Scientific Reports

Researchers from the 3SR Lab and their colleagues have released the first 3D high-resolution images of the human vocal folds thanks to X-ray microtomography coupled with a phase retrieval technique. These images reveal the complex structure of larynx tissues and open the way towards a better understanding of their virbo-mechanical properties.

Lucie BAILLY

3SR Lab


Image:Multiscale X-ray tomographic images of a human vocal fold. Left: global view. Center: detail of the tissue. Right: 3D reconstruction of the network of muscular, collagen and elastic fibres
Image:Multiscale X-ray tomographic images of a human vocal fold. Left: global view. Center: detail of the tissue. Right: 3D reconstruction of the network of muscular, collagen and elastic fibres

Human vocal folds possess the outstanding ability to endure large but reversible deformations and to reach vibration frequencies between 50 and 1 500 cycles per second. This unique performance mainly results from their complex specific 3D and multiscale structure. The characterisation of each element of this structure remains a great challenge today, even with the use of imaging techniques such as confocal microscopy (limited depth of field), magnetic resonance (limited spatial resolution) or X-ray absorption microtomography (limited phase contrast).

The researchers from the 3SR lab and their colleagues (1) have used high-resolution synchrotron X-ray microtomography associated to phase retrieval. Thanks to this technique, they have been able to report the first ex vivo 3D images of human vocal-fold tissues at multiple scales.

Various relevant descriptors of the structure were extracted from the images: the geometry of vocal folds at rest or in a stretched phonatory-like position, the shape and size of their layered fibrous architectures, the orientation, shape and size of the muscle fibres as well as the set of collagen and elastin fibre bundles constituting these layers.

The experimental methods used in this work open a promising way towards a better understanding of the micromechanics of the vocal folds, their deformation, and the link between the fibrous architecture and the vibratory properties.

This work should provide valuable guidelines for the design of new mimetic biomaterials for the next generation of artificial larynges.

 

(1) GIPSA-lab, LADAF, NOVITOM, ESRF

 

Ref: Bailly L, Cochereau T, Orgéas L, Henrich Bernardoni N, Rolland du Roscoat S, McLeer-Florin A, Robert Y, Laval X, Laurencin T, Chaffanjon P, Fayard B and Boller E: 3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode. Scientific Reports, Nature Publishing Group, 2018, 8 (1).

 

Read the full article online

Grafting antibiotics on cellulose for a new generation of strictly contact-active antimicrobial materials


Published in 2015 in Applied Materials & Interfaces

The researchers form the Laboratory for Pulp and Paper Sciences and their colleagues have achieved the first chemical grafting of penicillin directly on the surface of micro-fibrillated cellulose films, giving them unprecedented nonleaching and contact active antimicrobial properties.

Julien BRAS

LGP2


Image: pictorial representation of surface grafted micro-fibrillated cellulose film
Image: pictorial representation of surface grafted micro-fibrillated cellulose film

Micro-fibrillated cellulose is a bio-based material prepared from wood by a mechanical disintegration of the fibres. To exploit their amazing properties in the industry, one of the main challenges lies in the development of chemical modification processes aiming to increase the compatibility of micro-fibrillated cellulose with other materials or molecules, and produce a number of advanced materials with new functionalities.

Working in this way, the researchers from the Laboratory for Pulp and Paper Sciences have designed a new esterification reaction under aqueous conditions, to covalently bind the antibiotic penicillin directly to the surface of micro-fibrillated cellulose films. This process was proved efficient in grafting the antibiotic in a way that confers the film an antimicrobial effect occurring exclusively by contact, preventing any leaching or diffusion in the medium as demonstrated by their colleagues from the Paper Division of INNOVHUB (Milano, Italy). Such strictly contact-active antibiotic materials could find many applications, particularly in medical, food packaging or for the design of wound dressings.

Ref: Saini S, Belgacem N, Mendes J, Elegir G and Bras J, 2015 : Contact Antimicrobial Surface Obtained by Chemical Grafting of Microfibrillated Cellulose in Aqueous Solution Limiting Antibiotic Release. Applied Materials & Interfaces, 7, 18076-18085.

The amazing coils of the entangled wire


Published in 2016 in Nature Materials

The researchers from Laboratoire 3SR and their colleagues from ILM (Lyon) have designed a new material consisting in a single long coiled elastic or super elastic wire which showed unprecedented mechanical behaviour.

Laurent ORGEAS

3SR Lab


Image: X-Ray tomographic image of a NiTi entangled wire (sample height = 35 mm) showing the internal homogeneity of the material (Credit: 2015 Macmillan Publishers Limited)
Image: X-Ray tomographic image of a NiTi entangled wire (sample height = 35 mm) showing the internal homogeneity of the material (Credit: 2015 Macmillan Publishers Limited)

When squeezing a saturated wet sponge at low strain rates, its volume usually decreases causing the water to spill out. In the opposite direction, stretching the sponge will increase its volume inducing the sucking up of water. Most porous or dense materials actually behave this way, and a few rare types show the opposite behaviour, i.e. increase their volume when squeezed and decrease their volume when stretched.

However, for all these materials, the volume change is always symmetric, occurring in opposite directions under compression and tensile loadings. For the first time, researchers from the 3SR Lab and their colleagues from ILM Lyon have designed a mesoscale organisation of the matter able to break this “ancestral” symmetry, opening new material design possibilities for biomedical, mechanical or civil engineering applications.

Their porous material, made from a single entangled coiled wire (see image), actually expands whether being compressed or stretched. Moreover, the use of an elastic or a superelastic coiled wire confers the material the ability to bear repeated cycles of compression and tension without losing its amazing properties.

Thanks to discrete element simulations and mechanical experiments with 3D in situ observations using X-ray microtomography, they captured the complex mesomechanics of this material, which turn out to be a combination of the coiled wire's initial curvature, aspect ratio and level of entanglement as well as strain-induced coils deformation, all of which lead to an overall increase of the material porosity both in tension and compression.

Quite a weird sponge indeed, that could find many applications!

Ref: David Rodney, Benjamin Gadot, Oriol Riu Martinez, Sabine Rolland du Roscoat and Laurent Orgéas. Reversible dilatancy in entangled single wire materials. Nature Materials 15, 72–77 (2016).


Low intensity ultrasound treatment for high efficiency milk filtration


Published in 2014 in Journal of Membrane Science

The researchers from the Rheology Lab and their collaborators have demonstrated the efficiency of ultrasound to avoid membrane fouling during tangential ultrafiltration of skimmed milk.

Frédéric Pignon

LRP


Thanks to an original experimental design based on small angle X ray scattering (SAXS), the researchers form the Laboratory for Rheology and Processes and their collaborators have monitored the real-time evolution of casein micelle suspensions during cross-flow membrane filtration of skimmed milk.

At the nano-scale, they observed that over the process of filtration, a dense colloidal layer maintained by strong interactions between micelles was formed near the membrane. The formation of this viscous layer and its microstructure was studied and correlated to an important reduction of the transmembrane flow.

Interestingly, when applying low intensity ultrasound during the process, a clear disruption of the dense layer was obtained together with a significant increase in the permeation flux through the membrane.

Such unique results could find interesting applications in dairy industry, where cross-flow membrane filtration is widely used and encounters the major obstacle of irreversible membrane fouling.

Ref: Y. Jin, N.Hengl, F. Pignon, N. Gondrexon, M. Sztucki, G. Gésan-Guiziou, A. Magnin, M. Abyan, M. Karrouch, D. Blésès. Effect of ultrasound on cross-flow ultrafiltration of skim milk: characterization from macro-scale to nano-scale, J. Memb. Sci 470 (2014) 205-2018.


Grain of snow, or no grain of snow?


Published in 2014 in Granular Matter

By coupling experimental observations and mechanical modelling, the researchers from IRSTEA Grenoble have developed a new computational method to simplify the identification of individual snow grains in the complex network of ice crystals that makes up the snow.

Guillaume CHAMBON

IRSTEA ETNA


Image: 3D view of a snow sample (1 cm3). The bonds between snow grains were defined using either the method of mechanical stress computation (black rings) or the new algorithm based on geometrical clues (pink zones).
Image: 3D view of a snow sample (1 cm3). The bonds between snow grains were defined using either the method of mechanical stress computation (black rings) or the new algorithm based on geometrical clues (pink zones).

Snow is made of an assembly of sintered ice crystals forming a complex skeleton. At the microscale, the ice matrix has zones of mechanical weakness that delimit entities which can be viewed as snow grains. Rapid deformations of snow are mostly controlled by the dynamics of these mechanically-defined snow grains, their spatial rearrangements and contact interactions. When it comes to understanding the mechanical behaviour of snow in avalanche release processes for instance, the thorough identification of snow grains is of prime importance.

By comparing the distribution of mechanical stresses in snow samples and their 3D architecture, the researchers from IRSTEA have developed a new algorithm which uses local geometrical clues to infer zones of mechanical weakness in the ice matrix, and therefore simplifies the segmentation of the snow microstructure into grains.

When compared to the segmentation method based on the full computation of mechanical stress in the matrix, their algorithm enables a drastic reduction of the computational costs by a factor of 20. These results pave the way to a better consideration of the granular nature of snow in numerical simulations, in particular during rapid deformation phenomena where the large rearrangements of the microstructure render direct simulations extremely time consuming.

Ref: P. Hagenmuller, G. Chambon, F. Flin, S. Morin and M. Naaim, 2014: Snow as a granular material: assessment of a new grain segmentation algorithm. Granular Matter 16: 421-432.


Liquid micro-motors: the power of non-muscular cells


Published in 2015 in PNAS

By coupling experimental and numerical approaches, researchers from the Laboratory for Interdisciplinary Physics and their colleagues from Paris have recently elucidated the mechanisms by which a cell adapts its shape and regulates its traction forces to a substrate's stiffness.

Jocelyn ETIENNE

LIPhy DYFCOM


Image: Transmission microscopy image of a single cell adhering between two parallel microplates and submitted to a vertical traction force. The upper microplate is used as a nanonewton force sensor to measure the cell’s response (Credit: MSC Paris Diderot
Image: Transmission microscopy image of a single cell adhering between two parallel microplates and submitted to a vertical traction force. The upper microplate is used as a nanonewton force sensor to measure the cell’s response (Credit: MSC Paris Diderot

Living cells, just like muscles, exert forces on their surroundings. This mobility is at the heart of many biological processes such as cell division, embryogenesis, healing, infection, and immunity.

Although the molecules involved are of the same nature in muscles and non-muscle cells, their organisation is quite different: muscle cells have crystalline solid-like sarcomeres, whereas non-muscle cells have a disordered liquid-like actomyosin network.

A group of researchers from Grenoble and Paris have recently shown that in spite of these major differences, the key motor properties of muscles and cells result from similar mechanical phenomena. By comparing experiments and the predictions of a rheological model, they have been able to dissect and quantify the energy usage of a cell when pulling on its substrate, and to explain its amazing versatility and resilience regarding abrupt changes of its near environment.

Ref: J. Etienne, J. Fouchard, D. Mitrossilis, N. Bufi, P. Durand-Smet and A. Asnacios: Cells as liquid motors. Mechanosensitivity emerges from collective dynamics of actimyosin cortex. PNAS 112(9):2740–2745.


Undulations, waves, drops: from the virtual to the real


Published in 2013 in Journal of Fluid Mechanics

Researchers from the laboratory LEGI and their colleagues from the Virtual Assisted Atomisation Consortium have taken a big step towards the simulation of the complex flow instabilities occuring in gas assisted injection systems.

Alain CARTELLIER

LEGI


When a high speed gas flow impacts upon a liquid, we witness the formation of undulations, waves and droplet. Atomisation phenomena, commonly observed at the crests of waves, are a key principle of a number of propulsion systems. The efficiency and the reliability of such engines directly depend on the characteristics and the behaviour of the droplets produced.

By combining the results of controlled experiments with direct numerical simulations, researchers from the “Virtual Assisted Atomisation” consortium have developed a model to better understand where the droplets are formed and in what amount, as well as to calculate their size and velocity.

This leading-edge numerical code gives unprecedented access to the computation of severe flow conditions, equivalent to those encountered in gas assisted injection systems used in aeronautic propulsion. The VAA consortium is now actively working on the optimisation of the code with the aim of reducing the computational time.

Video: Air assisted atomisation of liquid films: experiments and simulations within the VAA project from the partner laboratories LEGI, IJLRDA, CORIA and IMFT


Ref: Fuster D, Matas JP, Marty S, Popinet S, Hoepffner J, Cartellier A and Zaleski S, Instability regimes in the primary breakup region of planar coflowing sheets, J. Fluid Mech., 736 150-176 (2013)


Light control of swimming algae: new perspectives for bioprocesses


Published in 2013 in Physical Review Letters

By studying the behaviour of swimming microalgae, the researchers from the laboratory LIPhy have highlighted an interesting photo focusing phenomenon that could serve some applications.

Salima RAFAI

LIPhy DYFCOM


Chlamydomonas reinhardtii is a swimming microalgae sensitive to light intensity. The researchers from the Interdisciplinary Laboratory of Physics in Grenoble studied the mechanical behaviour of a suspension of these algae in a pipe stream. They showed that the microorganisms will spontaneously migrate and concentrate around the center of the flow when stimulated by a light source placed upstream.

This focusing phenomenon was demonstrated to be due to the interactions between the algae movements towards the light source and the vorticity of the flow.

Considering the important potential of these microorganisms in bio-conversion, this result represents the first step towards new algae separation techniques, a major obstacle in bio-production processes. In an environmental perspective, the presence of certain pollutants was observed to significantly alter the phototaxis of these microswimmers, reducing their self-focusing ability. They could therefore be used in bio-sensors for the accurate detection of water contaminants.


Ref: Garcia X, Rafaï S, Peyla P (2013) Light Control of the Flow of Phototactic Microswimmer Suspensions. Phys. Rev. Lett.


Four dimensional characterisation of sand deformation


Published in 2012 in Acta Geotechnica

For the first time, the researchers from the 3SR lab were able to observe the motion of fifty thousand sand particles in 3D using x-ray micro-tomography. 

Edward ANDO

3SR


Image: 3D view from x-ray microtomography of a granular assembly (grey-scale) overlayed with measured 3D grain rotation angles (colour-scale) during axial loading
Image: 3D view from x-ray microtomography of a granular assembly (grey-scale) overlayed with measured 3D grain rotation angles (colour-scale) during axial loading

Each particle in the sample is identified by its 3D geometry, and its kinematics (translation and rotation) are measured at every 1% shortening of the sample (around 0.22 mm) while it is deformed. This technique opens the way towards the understanding of strain localization and global deformation in granular materials through the dynamics occurring at the particle scale.

Ref: Andò E, Hall S A, Viggiani G, Desrues J, Bésuelle P (2012) Grain-scale experimental investigation of localised deformation in sand: a discrete particle tracking approach. Acta Geotechnica.


3D X-ray imaging of biofilm deposition


Researchers from the 3SR lab have obtained the first 3D images of a biofilm, formed on a fixed bed of biolite beads.

Sabine ROLLAND DU ROSCOAT

3SR Lab


Image: three dimensional view of a biofilm (purple) on biolite beads (yellow) obtained at the ESRF facility
Image: three dimensional view of a biofilm (purple) on biolite beads (yellow) obtained at the ESRF facility

This development opens up possibilities to better understand processes which rely on bio filtration or any other area which is affected by biofilm deposition.